Fig. 6. Long-term effect of allicin on DNA methylation. (A) Dot blot analysis of 5-methyl cytosine (5'mC) and 5-hydroxymethyl cytosine (5'hmC) in A549 cells treated with 2μg/ml allicin and cultured in normoxia for 7days and 14days (B) mRNA quantification of Tet1, Tet2, Tet3 genes by real-time PCR in untreated and the 2μg/ml allicin treated A549 cells. Results indicate fold change relative to untreated cells after normalization to the 18s expression. The bars represent the mean ± standard deviation calculated from three independent experimental values. UT= untreated.